Error running SpaceRanger Workflow from lilab-bcb/cumulus

Post author
Rômulo Galvani

After inserting the inputs for samplesheet and outputs in the format "gs://...", backend as "gcp", memory as "120G", num_cpu as 32, run_count as true, run_mkfastq as false (my files are fastq), secondary as true, spaceranger_version as "2.0.0" or other I receive failure with the following message "job spaceranger_workflow.generate_count_config:NA:1 exited with return code 1 which has not been declared as a valid return code". Does anyone know how to help?

Comments

3 comments

  • Comment author
    Pamela Bretscher

    Hi Rômulo,

    Thank you for writing in about this issue. Can you share the workspace where you are seeing this issue with Terra Support by clicking the Share button in your workspace? The Share option is in the three-dots menu at the top-right.

    1. Toggle the "Share with support" button to "Yes"
    2. Click Save

    Please provide us with

    1. A link to your workspace
    2. The relevant submission ID
    3. The relevant workflow ID

    We’ll be happy to take a closer look as soon as we can!

    Kind regards,

    Pamela

    0
  • Comment author
    Rômulo Galvani

    Workflow link:

    Lung Einstein - Dashboard (terra.bio)

     

    Submission ID:
    d0407352-ddcd-412d-ae0d-0cfe2c246168

    Submission Link: 
    https://console.cloud.google.com/storage/browser/fc-05ff183a-e254-4336-8f11-54b553be94d1/submissions/d0407352-ddcd-412d-ae0d-0cfe2c246168?authuser=ayzrael@gmail.com

    Workflow ID:
    264c10fe-80ba-4c2a-871a-fcb847c013ab

     

    Thank you in advance

    0
  • Comment author
    Pamela Bretscher

    Hi Romulo,

    Thank you for providing that. When looking at the backend log for the failed workflow, it looks like the workflow is failing with the following error that indicates an issue with the "Flowcell" column of your input CSV. 

    In your input CSV, it looks like you just have the same link to the main workspace bucket path for each sample in the "Flowcell" column. From the SpaceRanger documentation, it looks like this column should contain different Google bucket URLs for BCL folders. 

    Please let me know if this information is helpful.

    Kind regards,

    Pamela

    0

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